SOLUBLE EXPRESSION OF ? AND ? GENES OF HUMAN HEMOGLOBIN IN ESCHERICHIA COLI
- VernacularTitle:人血红蛋白?、?基因以可溶形式在大肠杆菌中的表达
- Author:
Hao ZHANG
;
Quanli WANG
;
Bingzhi MAO
- Publication Type:Journal Article
- Keywords:
escherichia coli;
gene expresion;
globin
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Fragments of ? and ? genes were cloned into expression plasmid pET-21b. Bacteria harbouring expression plasmid was induced with IPTG,and a band of expressed protein of 16 kD was found in PAGE. They expressed as soluble products. ? expression product reached about 5% of total bacterial protein,? expression product reached about 15% of total bacterial protein. The products were confirmed by Western-blotting.
