Study and application of immunogold filtration assay for detecting serum antibodies against helicobacter pylori.
- VernacularTitle:胶体金免疫渗滤法快速检测幽门螺杆菌血清抗体的研究和应用
- Author:
Ruishu ZHU
;
Rui ZHOU
;
Bo LU
- Publication Type:Journal Article
- Keywords:
Helicobacter pylori Staphylococcal protein A(SPA) Immunogold filtration assay
- From:
Chinese Journal of Practical Internal Medicine
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To establish a simple,quick,accurate serodiagnostic assay for detecting serum antibodies against Helicobacter pylori(H.pylori).Methods We established quick immunocolloidal gold filtration assay(DIGFA) by combining colloidal gold labelling technique with filtration method.145 sera from cases with H.pylori infection were detected by DIGFA,and the results were compared with that of ELISA and pathological examinations.In addition,blocking test and human IgG protein detection were conducted.The sensitivity,specificity of DIGFA were analysed.Besides,DIGFA was applied to investigate the H.pylori infection situation among persons of different ages.Results The coincidence rate reached 95 1% compared with the ELISA,and it was 88 8% compared with pathological the examination.The detectable minimum amount of IgG was 10?g/mL.The antibodies detected by DIGFA proved to be specific antibodies by blocking test.The human H.pylori infection rate among 216 persons reached 42 5% by DIGFA.Conclusions DIGFA can be applied effectively in clinical diagnosis and epidemiological survey.
