Cloning and sequence analysis of 4 amastin genes from Leishmania parasites
- VernacularTitle:利什曼原虫无鞭毛体蛋白的基因克隆化与序列分析
- Author:
Jun CHENG
;
Yanwei ZHONG
;
Yan LIU
- Publication Type:Journal Article
- Keywords:
Leishmanina major;
Trypanosome;
Gene cloning;
Amastin
- From:
Chinese Journal of Infectious Diseases
1999;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To clone amastin coding genes from different strains of Leishmania parasites. Methods Using amastin cDNA sequence as the reference, dbEST data base established by National Center Biotechnology Information (NCBI), USA, was searched by BLAST tool. A 309 bp DNA fragment of Leishmania major was found and used as the probe for the screening of a DNA library. The amastin gene of Leishmania major Abdou was cloned and sequenced. Specific primers were designed and amastin genes for Leishmania mexicana WR972, Leishmania brizeliensis and Leishmania amazonensis joseph were amplified by polymerase chain reaction. Results The amastin genes from 4 strains of Leishmania parasites were cloned and sequenced. It was found that all 4 amastin genes contained unique open reading frame of 552 bp and encoded amastin protein of 183 amino acid residues. Conclusion The amastin genes of 4 strains of Leishmania parasites were successfully cloned.
