EFFECTS OF EXOGENOUS PTEN GENE ON THE CELL CYCLE AND PROLIFERATION OF SHG44 GLIOMA CELL
- VernacularTitle:外源性PTEN基因稳定转染对SHG44细胞周期和增殖的影响
- Author:
Xia LI
;
Xiang ZHANG
;
Jingwe WU
- Publication Type:Journal Article
- Keywords:
glioma;
PTEN;
gene therapy
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Eukaryotic expression vector containing PTEN gene was transfected into SHG44 cell under mediation of lipofectamine, and positive cell clones were selected and amplified. Expression of PTEN gene was detected by in situ hybridization and immunohistochemistry. Flow cytometry, electron microscope and other methods were adopted to measure the effects of PTEN on cell cycle, ultrastructure, and cell growth of the transfected glioma cells, as well as on tumorigenicity in nude mice. The results showed that PTEN gene and protein were expressed in the glioma cells transfected with PTEN gene. The progression of cell cycle was arrested from G1 to S phase. The growth of cells transfected with PTEN gene in nude mice was dramatically inhibited compared with the parent SHG44 cells. This experiment suggested that the growth of SHG44 glioma cells could be arrested at G1/S phase, and it could be significantly suppressed by exogenous PTEN gene.
