INDUCTION OF APOPTOSIS OF HUMAN LIVER CARCINOMA CELLS BY TEA POLYPHENOLS
- VernacularTitle:茶多酚诱导肝癌细胞凋亡
- Author:
Qin WU
;
Tiquan FU
;
Chuanqian FENG
- Publication Type:Journal Article
- Keywords:
tea polyphenols;
carcinoma,hepatocellular;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Tea polyphenols were tried to induce apoptosis of human cultured hepatocellular carcinoma cell line HepG Ⅱ. MTT assay, DNA agarose gel electrophoresis, transmission electron microscopy , fluorescence decoration and DNA end labeling method (Tunel) were used to identify apoptosis. Having been treated by tea polyphenols in 250?g/ml , HepG Ⅱcell apoptosis was induced. The induction of apoptosis was the dose dependent. Chromatin condensation, apoptotic body formation, fluorescence of yellow green and pellet were observed. Agarose gel electrophoresis analysis revealed DNA cleavages( DNA ladder). The results indicated that tea polyphenole could induce apoptosis of cultured human hepatocellular carcinoma cell line HepG Ⅱ. It suggested that tea polyphenols could be used to treat human liver carcinoma.
