DETECTION OF HIV CORECEPTOR CCR5 MUTATION IN UIGUR POPULATION FROM SINKIANG UIGUR AUTONOMOUS REGION
- VernacularTitle:新疆维吾尔族人群HIV协同受体CCR5基因突变的检测
- Author:
Mingxu LIU
;
Fusheng WANG
;
Le JIN
- Publication Type:Journal Article
- Keywords:
HIV 1 coreceptor;
CCR5?32;
allelic polymorphism;
PCR RFLP
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Whole blood samples were collected from 316 Uigur subjects inhabiting Kashi City, Sinkiang Uigur Autonomous Region, and their genomic DNA was extracted using QIAgen Blood Kit. DNA fragment spanning CCR5?32 and CCR5m303 mutation points were amplified by PCR and genotyped by directed electrophoresis and RFLP with HinCII respectively. In all 316 samples only 22 heterozygotes of CCR5?32 were found, in accordance with Hardy Weinberg equilirium. No CCR5m303 mutation was found. No difference existed between male and female individuals. The frequency of CCR5?32 in Uigur population is 3.48%, similar to Medi Asia ethnic groups (higher than that of Chinese Han people and lower than that of Caucasian people), i.e, in continuity regarding district distribution. Due to its slow down effect on clinical course of AIDS , high frequency of CCR5?32 is highly significant in prevention and treatment of AIDS in Uigur population.
