PREPARATION OF MONOCLONAL ANTIBODY AGAINST THE DEGRADATION FRAGMENT OF FIBRONECTIN (MAD2)AND DETECTION OF SERUM MAD2 IN THE PATIENTS WITH HEPATOCELLULAR CARCINOMA
- VernacularTitle:纤维连接蛋白降解片段(MAD2)单抗的制备及肝细胞癌患者血浆含量检测
- Author:
Jingmin ZHAO
;
Shouchun YANG
;
Yedon WANG
- Publication Type:Journal Article
- Keywords:
liver neoplasm;
fobronectin;
monoclonal antibody
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
In order to develop ELISA method for measuring the degradation fragment of fibronectin (MAD2),hybridoma technique was used to obtain the monoclonal antibody (McAb)IgG 1 against MAD2 without cross reaction with fibronectin.The sera from 277 patients with hepatocellular carcinoma (HCC),76 with metastatic hepatic carcinoma (HMC),98 with alimentary canal carcinoma (ACC) and 156 with chronic liver disease(CLD) and 48 healthy subjects were assayed with ELISA method using this antibody.The examination showed that the mean value of MAD2 from the patients with HCC showed obviously significant difference compared with those of CLD,HMC,ACC and normal control groups ( p
