THE EXPRESSION OF FUSION PROTEIN AFTER INJECTION OF PSP94-TNF?D11a PLASMIA DNA IN MICE
- VernacularTitle:PSP94-TNF?D11a质粒DNA注射体内表达规律
- Author:
Qingxin LIU
;
Li LIU
;
Lizhon LIU
- Publication Type:Journal Article
- Keywords:
prostatic carcinoma:gene theraphy;
plasmid intramuscular injection
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
PSP94 TNF?D11a naked plasmid intramuscular injection on intact mice.PSP94 TNF?D11a cDNA cloned in pcDNA3,an eukarocytic experssion plasmid,was intramuscularly injected into the quadriceps muscle of 39 mice (50?g each). Serum was collected .The expression of fusion protein was detected by ELISA.Uninjected mice were used as control.RNA of PSP94 TNF?D11a from tissue of skeletal muscle was determined using reverse transcription polymerase chain reaction analysis.The result showed that the levels of expressed protein were remarkably elevated in the serum nine days after the injection of naked plasmid DNA ,peaking on the fourteen days ,and maintaining at a detectable level up to 25 days.
