Detecting Chromosome Aberrance of Oral Squamous Cell Carcinoma (OSCC) in Cytogenetics.
- VernacularTitle:应用细胞遗传学方法检测口腔鳞状细胞癌染色体畸变
- Author:
Kan LI
- Publication Type:Journal Article
- Keywords:
Squamous cell carcinoma;
Chromosome
- From:
Journal of Medical Research
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Detect chromosome aberrance(number and construction) in OSCC cell .Methods Cell were treated with colchicines and microsometic fluid, and then were expanded with cold acetic acid and formalized with methyl alcohol. After Giemsa staining, the cell were observed under oil microscope and the chromosome karyotype was analyzed. Results The number of OSCC chromosome is between 63~66, and many type of chromosome contruction is aberrant, chromosome break and monocase of chromosome break. The number change is appear in increasing of NO 1、7、16、19、20 and lose in NO 6、9、10、18、21、22, the constructing change in NO 1、2、4、11. Conclusion Analysising chromosome aberrance will be useful in location in chromosome for relative oncogene and suppressor gene.
