Effect of isoflurane on pulmonary surfactant synthesis in cultured primary alveolar type Ⅱ cells
- VernacularTitle:异氟醚对原代培养的肺泡Ⅱ型上皮细胞合成表面活性物质的影响
- Author:
Yongwang LI
;
Tiande YANG
;
Qiaoyi LIU
- Publication Type:Journal Article
- Keywords:
isoflurane;
pulmonary surfactant;
alveolar type Ⅱ cells
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine the effect of isoflurane(Iso) on pulmonary surfactant(PS) synthesis in cultured primary ATⅡ cells. METHODS ATⅡ cells were isolated from adult rat lungs and used for the experiments after 32 h in primary culture. Iso 0.28 and 2 8 mmol?L -1 was added into the media of normal and H 2O 2 (75 ?mol?L -1 )-treated cells, and the cells were further incubated for 2 h. The cell proliferation was measured with MTT method and PS synthesis with 3H-choline chloride incorporation. RESULTS Iso had no effect on the proliferation of ATⅡ cells, but markedly decreased PS synthesis in normal alveolar type Ⅱ cells, and aggravated the decrease of PS synthesis induced by H 2O 2. CONCLUSION Iso may decrease PS synthesis of alveolar type Ⅱ cells in vitro , and aggravate the damage of the cells under peroxidation condition.
