Protective effects of polydatin on mouse hepatocyte injury induced by hydrogen peroxide in vitro
- VernacularTitle:白藜芦醇苷体外对过氧化氢导致小鼠肝细胞损伤的保护作用
- Author:
Zhixian MO
;
Hongxia SHAO
- Publication Type:Journal Article
- Keywords:
polydatin;
hydrogen peroxide;
hepatocyte;
antioxidative effe
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of polydatin on mouse hepatocyte injury induced by hydrogen peroxide(H 2O 2). METHODS Mouse hepatocytes were prepared. SOD activity, MDA content, GSH level, GSH-px activity, glutamic-pyruvic transaminase (ALT), nitric oxide(NO) and nitric oxide synthase(NOS) were determined with different method, respectively. RESULTS After 1 h incubation, the mouse hepatocytes with various concentrations of polydatin (0 05, 0 1, 0 5, 1, 2, 4 mmol?L -1 ), significantly decreased the contents of NO and MDA, inhibited the NOS activity, increased the activities of SOD and GSH-px, suppressed the consumption of GSH, and reduced the elevated ALT level in the supernatant induced by H 2O 2. CONCLUSION Polydatin can protect hepatocytes from oxidative injury induced by H 2O 2.