Effects of magnesium salicylate (MgS)on free intracellular calcium in isolated embryonic rat brain cells
- VernacularTitle:水杨酸镁对胎鼠脑细胞内钙的影响
- Author:
Yueqin LIANG
;
Xuanping ZHANG
;
Yunzhao TANG
- Publication Type:Journal Article
- Keywords:
magnesium salicylate;
calcium antagonist;
Fura-2/AM;
intracellcular free calc|
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the calcium antagonistic effects of Magnesium salicylate in central nervous system. MTEHODS Using calcium sensitive fluorescence indicator Fura-2 technique. RESULTS Resting [Ca 2+ ] i in brain cells under different extracellular calcium concentration(0,0 01,0 1,1 0,1 3 mmol?L -1 )were 41?5,59?6,84 7?2 5,104?7,(126?5)nmol?L -1 respectively( n =8～47).MgS 0 2 mmol?L -1 had no significant effects on resting [Ca 2+ ] i.At the same time, MgS (0 05～0 4 mmol?L -1 ) concentration dependently inhibited the [Ca 2+ ] i elevation induced by high extracellular potassium or L -glutamate in the presence of extracellular [Ca 2+ ] i 1 3 mmol?L -1 . The IC 50 values were 0 323 mmol?L -1 (95% CI 0 122～0 854 mmol?L -1 ) and 0 124 mmol?L -1 (95% CI 0 073～0 210 mmol?L -1 ) separately. CONCLUSION MgS possesses inhibitory effects on voltage-activated calcium channel and receptor-operated calcium chanel in CNS.
