The in vivo gene transfer efficacy and expression patterns by hepatic artery administration of recombinant adenovirus
- VernacularTitle:经肝动脉插管注射重组腺病毒的体内基因转染效率及“靶向性”的研究
- Author:
Qiang HAO
;
Jianming TIAN
;
Xuetao CAO
- Publication Type:Journal Article
- Keywords:
Animal testing alternatives;
Radiology,interventional;
Catheterization;
Gene transfer;
Transfection;
Adenoviridae;
Biological markers;
Gene expressiX
- From:
Chinese Journal of Radiology
1999;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the gene transfer efficiency and lasted time in rat organs by hepatic artery injection with LacZ reporter gene recombinant adenoviruses. Methods Seven groups of rats were injected with Ad.LacZ (2?10 9 pfu/ml) and two groups of rats were injected with PBS 1 ml as control separately through gastra intestinal artery, and liver, spleen, lung, and kidney were gotten at 12 hrs, 18 hrs, 72 hrs, 7 day, 14 day, 21 day, and 28day, respectively. X gal staining was used to check up expression of LacZ gene. Results Expression of LacZ gene was detected in liver 12 hrs after injection, but none were done in spleen, lung, and kidney. Up to 21days, LacZ gene expressed in liver, but the gene expression lasted for only 14 days in spleen, lung, and kidney LacZ gene was not detected in the two control groups in all organs at 7 day. Conclusion When recombinant adenovirus was administrated through hepatic artery, the introduced gene expressed preferentially in liver. This result was the basis of intraarterial administration of cytokines gene to treat liver tumor.
