Treatment of metastatic liver cancer in rat by hepatic artery injection of cytokine recombinant adenoviruses
- VernacularTitle:经肝动脉插管注射重组腺病毒治疗大鼠转移性肝癌的实验研究
- Author:
Qiang HAO
;
Jianming TIAN
;
Xuetao CAO
- Publication Type:Journal Article
- Keywords:
Animal testing alternatives;
Radiology, interventional;
Interleukin 2;
Tumor necrosis factor;
Genetic vectors;
Adenoviridae
- From:
Chinese Journal of Radiology
1999;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the therapeutic effects of TNF and IL 2 recombinant adenoviruses via intra arterial injection on metastatic liver cancer in rat model. Methods Recombinant adenoviruses harboring hTNF ? or hIL 2 gene were amplified in 293 cells and subjected to titration by the pathogenetic effects on 293 cell. The rats bearing metastatic liver cancer of Walker 256 breast carcinoma were randomly grouped and administered via gastra intestinal artery with hTNF ? recombinant adenoviruses alone, or hIL 2 recombinant adenoviruses alone, or at the dose of 1.0?10 9 pfu/rat. The therapeutic effects were observed including their survival time. Results The prepared recombinant adenoviruses of hTNF ? and hIL 2 were with the titers of 2.0?10 9 pfu/ml and 2.1?10 9 pfu/ml, respectively. 1.0 ?10 9 pfu hTNF was the proper dose. Administration of hTNF ? or hIL 2 recombinant adenoviruses via hepatic artery could extend the survival time of metastatic liver cancer bearing rats, with the better therapeutic effects achieved by combinatorial administration of these two adenoviruses. Conclusion Arterial administration of adenoviruses may be an effective approach to targeted immunogene therapy for cancer.
