Inhibition of transdifferentiation of human renal tubular epithelial cells by valsartan
- VernacularTitle:缬沙坦抑制人类肾小管上皮细胞转分化的初步研究
- Author:
Xiaoyan WEN
;
Falei ZHENG
;
Yang SUN
- Publication Type:Journal Article
- Keywords:
Renal tubular epithelial cells;
Transdifferentiation;
Myofibroblast;
Valsartan
- From:
Chinese Journal of Nephrology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible role of valsartan(Val) in transdifferentiation of human renal tubular epithelial cell line(HKC ). Methods HKC cells were divided into four groups: (1) serum-free (negative control); (2) MCP-1 + AAI-treated (positive control); (3) Val-treated alone; (4) Val-inhibition(treated with MCP-1 + AAI + Val). Then the expression of vimentin, ?-smooth muscle actin (?-SMA ) of HKC cells were assessed by indirect enzyme immunohistochemistry (IEI), and the percentage of ?-SMA(+ ) HKC cells was assessed by flow cytometry. Results No difference in expressions of vimentin and ?-SMA by IEI and the percentage of ?-SMA(+ ) HKC cells by cytometry were found between serum-free control HKC cells and Val treated ones. The expressions of vimentin and ?-SMA in positive controls were markedly stronger than negative controls; while these expressions in Val + MCP-1 + AAI-treated HKC cells were less strong than those in positive controls. The percentage of ?-SMA(+ ) HKC cells in the positive controls was significantly higher than that in negative controls(91. 8% vs 3. 1%, P
