Inhibitory effect of IL-10 on induced expression of ICAM-l in renal tubular cells is mediated by down regulated activity of nuclear factor kB
- VernacularTitle:白细胞介素10抑制核因子kB介导的肾小管上皮细胞表达细胞间黏附分子1
- Author:
Jianliang CHEN
;
Xiaomei LI
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Interleukin-10;
Renal tubule;
Epithelial cell;
Interecllular adhesion molecule-l;
Transcription factor
- From:
Chinese Journal of Nephrology
1997;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objecive To explore the effect of interleukin-10(IL10) on tumor necrosis factor-?(TNF?)- induced expression of intercellular adhesion molecule 1 (ICAM-1 )and related transcription pathway in human renal tubular cells. Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. ICAM-1 was measured by cell ELISA and Northern blot in protein and mBNA levels. Activities of transcriptional factors nuclear factor GB(NFkB) and activator protein 1 (AP-1) were determined by electrophoresis mobility shift assay. Results TNF?-induced ICAM-l expression in HK-2 cells was increased in both protein and mNRA levels(P
