The role of nitric oxide and nitric oxide synthase in killing tumor cells
- VernacularTitle:一氧化氮及其合酶在杀伤肿瘤细胞中的作用
- Author:
Chong BAI
;
Yiping HAN
;
Zhongling LIU
;
Shiming ZHANG
- Publication Type:Journal Article
- Keywords:
macrophages, alveolar;
nitric oxide synthase;
neoplasms
- From:
Academic Journal of Second Military Medical University
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the role of inducible nitric oxide synthase (iNOS) in activated macrophages and nitric oxide (NO) in the defence against tumors. Methods: Macrophages were obtained by alveolar lavages of mice and activated through incubation with recombinant murine INF? in vitro. P815 cells were added to the culture. Culture supernates were collected to measure the activity of iNOS and NO. Tumoricidal activity of macrophages was determined in presence and absence of the specific inhibitor of NO synthase: L NMMA. Results: NO production and activity of iNOS induced by activated macrophages were positively related with concentration of INF? in macrophage P815 coculture. The addition of L NMMA to the culture suppressed NO production, the inhibitory rate of activated macrophages against P815 cells was reduced distinctly ( P
