The expression of transforming growth factor ?_1 in renal cortex from uninephrectomized diabetic rats
- VernacularTitle:转化生长因子?_1在单侧肾切除糖尿病大鼠肾皮质中表达
- Author:
Yonggui WU
;
Shanyan LIN
;
Yi YU
- Publication Type:Journal Article
- Keywords:
Transforming growth factor ?_1 Kidney enlargement Angiotensin converting enzyme Extracellular matrix
- From:
Chinese Journal of Nephrology
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of transforming growth factor ?_1(TGF?_1)in renal cortex from uninephrectomized diabetic rats. Methods Wistar rats were divided into uninephrectomized rats(group A), streptozotocin diabetic rat(group B). Blood glucose, serum insulin level and body weight, kidney weight, kidney weight/body weight as well as renal tissue protein contents were observed after 1, 4 weeks of streptozotocin injection. The expression of TGF?_1, precollagen 1?(Ⅳ) and fibronectin mRNA were measured by Northern blot analysis, and TGF?_1 protein by Western blot analysis in kidney cortex. In addition, ACE activities were determined by fluorimetric assay in plasma, kidney cortex and medulla. Results Group B demonstrated significantly elevated blood glucose and decreased serum insulin level. Kidney weight、kidney weight/body weight and renal tissue protein contents progressively increased despite total body weight loss. There was significant(P
