Construction of retroviral vector carrying human IL-3 cDNA under control of human AFP enhancer core sequence and SV40 early region promoter
- VernacularTitle:携带HuIL-3基因的人肝癌特异性逆转录病毒载体的构建
- Author:
Jun GAO
;
Guangwen CAO
;
Zhongtian QI
;
Ping DU
;
Xiaofang QIU
;
Wenguo YANG
;
Long CUI
;
Rumei SUN
- Publication Type:Journal Article
- Keywords:
interleukin-3;
liver neoplasms;
retroviral vectors;
gene therapy
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To construct retroviral vector carrying human interleukin-3 c0mplementary DNA(HuIL-3 cDNA) under control of human a-fetoprotein gene enhancer core sequence and human SV4O pro-moter. Methods and Results: HuIL-3 cDNA was inserted into polylinker site of retroviral vector pMNSMto construct retr0viral vector pMNS-IL-3, in which the transcription of HuIL-3 cDNA was drived by SV40early region promoter. Human Q-fetoprotein gene enhancer core sequence was released from plasmidpGEM. 7Zf-AFPe and inserted into the polylinker site of pMNSM. Then human interleukin-3 cDNA wasinserted int0 p0lylinker site to construct retroviral vector pMNSA-IL-3, in which HuIL-3 cDNA transcrip-tion was drived by SV40 early region promoter and enhanced by human a-fetoprotein enhancer core se-quence- Conclusion: The vectors are of significance for hepatoma-specific gene therapy.
