Tissue-specific regulation of vitamin D_3 receptor mRNA expression by 1, 25-dihydroxyvitamin D_3
- VernacularTitle:1,25-二羟维生素D_3对其D_3受体mRNA表达的细胞特异性调节
- Author:
Liangnian SONG
- Publication Type:Journal Article
- Keywords:
1 , 25-dihydroxyvitamin D_3;
receptor, 1, 25-dihydroxyvitamin D_3;
mRNA;
gene expression;
RT-PCR
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
To explore the potential regulatory effects of 1, 25-dihydroxyvitamin D, L1' 25 (0H),D,j on the expression of vitamln D, receptor (VDR ) mRNA in human megakaryoblastic leukemia(HIMeg) and human osteosarcoma (HOS-86O3) cell lines. Methods: VDR mRNA expression was deter-mined by the utilization of our recently established quantitative reverse-transcripti0n p0lymerase chain re-acti0n (RT~PCR) method. Results: lt was found that VDR mRNA expression in HOS-86O3 cells was notchanged significantly after treatment with 1, 25 (OH),D, for O~ 24 h- On the other hand, treatment 0fHIMeg ce1ls with 1, 25 (OH),D, cou1d significantly induce a d0wn-regulati0n of VDR mRNA expressi0n ina time-dependent manner, reaching a level of about only 15% of control after 24 h treatment. C0ncIusi0n:The regulation of VDR mRNA expressi0n by 1,25 (OH), D, is of tissue-specific fashi0n.
