Ralationship between potential doubling time(Tpot) and repopulation of clonogenic cells for human nasopharyngeal carcinoma
- VernacularTitle:人鼻咽癌细胞CNE潜在性倍增时间(Tpot)与其放射后再增殖
- Author:
Jie ZHU
- Publication Type:Journal Article
- Keywords:
Potential doubing time radiation effects
- From:
Chinese Journal of Radiation Oncology
1995;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.
