Reverse phase HPLC analysis of N-ATRP and its metabolite TRP in rabbit serum
- VernacularTitle:反相HPLC法测定家兔血清中N-乙酰色氨酸及其代谢产物
- Author:
Weiren XU
;
Yuefang WEI
- Publication Type:Journal Article
- Keywords:
N-ATRP;
TRP;
pharma-cokinetics;
reversed phase HPLC;
fluorescence detection
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
A simple, rapid and sensitive method for reversed phase HPLC determination of N-acetyl-L-tryptophan (N-ATRP) and its metabolite L-tryptophan (TRP) in rabbit serum was developed based on their intrinsic fluorescence using excitation and emission wavelengths of 280 and 360 nm, respectively. The separation of two amino acids was achieved on an ODS C18 column with a mobile phase of 0. 1 mol ?L-1 NaAc-25% methanol (pH 5. 7) at a flow rate of 0. 5 ml ?min-1. The calibration curves for N-A-TRP and TRP provided the linear range about1.0 - 17. 0mg ?L-1(r=0. 9962 and 0.9978, respectively). The recoveries greater than 98% and good reproducibility were achieved with rabbit serum. The concentration-time curve of N-ATRP after iv administration was determined in rabbits. Results showed that N-ATRP was quickly converted to TRP with a half-life of 8. 5 min. The decrese of N-ATRP accorded well with the rise of TRP.
