ESTABLISHMENT AND PRELIMINARY APPLICATION OF DOT COLLOIDAL DYE IMMUNOASSAY FOR ANTIBODY DETECTION IN PATIENTS OF SCHISTOSOMIASIS JAPONICA
- VernacularTitle:胶体染料斑点免疫试验检测血吸虫病人抗体方法的建立及初步应用
- Author:
Yinchang ZHU
;
Chuanxin YU
;
Xuren YIN
- Publication Type:Journal Article
- Keywords:
colloidal dye,schistosomiasis japonica,antibody detection
- From:
Chinese Journal of Schistosomiasis Control
1989;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
A kind of colloidal blue dye(D-l)used as a staining reagent for immunoassay was first selected from the dyes produced in China in this study. The optimun condition for labelling the dye onto sheep antihuman IgG antibody was explored. The dye-labelled antibody could react and stain with relative antigens. The minimal concentration of human IgG protein could be detected with the labelled dye by Dot Double Antibody Sandwich Assay was 20-10ng/ ml. 61 of 62 sera samples of schistosomiasis japonica were positive,the positive rate was 98.4%. Just 1 out of 30 healthy people's sera sambles was positive,the false positive rate was 3.3%. All of 40 Fasciolopsiasis sera samples were negative. The results were similar to that of Dot-ELISA. The activity of antibody labelled with colloidal dye could be maintained for 1 week in room temperature and be presered in lyophilized condition for a longer period. The assay was less expensive,simple to conduct and required no special equipment. It suggested that the Dot Colloidal Dye Immunoassay(DIA)might have potential value for use in schisto-somiasis endemic areas.
