DETERMINATION OF ?-CAROTENE IN VEGETABLES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
- VernacularTitle:蔬菜中?-胡萝卜素的反相液相色谱测定法
- Author:
Fengqi XIONG
;
Lujiang YUAN
- Publication Type:Journal Article
- Keywords:
reversed-phase HPLC gradient elution ?-carotene
- From:
Acta Nutrimenta Sinica
1956;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
?-carotene of vegetables was extracted with petroleum ether and ether (1:1). The extract was evaporated at 45℃ in water bath. The residue was dissolved in n-hexane and analysed on a column of?Bondapak C18 with gradient elution, methyl alcohol as mobile phase. It was then detected at 450nm by spectrophotometer. The retention time of ?-carotene was 13.71min. The recoveries were 94.1% to 102.2%. The coefficient of variation was 2.38% to 5.17%. The method was simple and rapid.
