Effects of Three Kinds of Mutagens on the Cell Survival Rate and the Chromosomal Aberration in V79 Cells
- VernacularTitle:三种诱变剂对V79细胞及其突变株存活率和染色体畸变的影响
- Author:
Donglian CAI
;
Zheng NIE
;
Nan LIU
;
Jun QIAN
- Publication Type:Journal Article
- Keywords:
N-methyl-N'-nitro-N-nitriosoguanidine;
mitomycin C;
ethyl methanesulphonate;
V79 cells;
chromosomal aberration
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The cell survival rate and the chromosomal aberration induced by N-methyl-N-nitro-N-nitriosoguanidine (MNNG), mitomycin C (MMC) and ethyl methanesuiphonate (EMS) in V79 cells and its ?-ray-sensitive mutants (irs5 and mil) were observed in this study. The concentrations of mutagens to determine the survival rate were arranged at 2.5~ 30 ?mol/L in MNNG, 1~20?mol/L in MMC and 2~ 16 ?mol/L in EMS, respectively. The cell survival rates and the cell clones of irs5 were higher than that of the V79 cells and irs11; and of irs11 were significantly lower than that of V79 cells and irs5. The result of IC50 of mutagens to determine the chromosomal aberration was similar to the cell survival rate. The type and frequency of chromosomal aberration induced by MNNG, MMC and EMS in irs5 was same as in V79 cell; and there was a dose-response relationship between them. The spontaneous aberration frequencies in irsS and V79 cells were lower than 4%, however, in irs11 was higher than 10%. Data suggests that the cellular biological characterization in irs5 is steady, and further study to certain irsS whether it will be a new mutant for the mutagenicity or carcinogenicity assay or not.
