The Induction of hsp70 mRNA under Heat Stress
- VernacularTitle:热应激对热休克蛋白70mRNA的诱导
- Author:
Bingyi WU
;
Liangnian SONG
;
Renbao XU
- Publication Type:Journal Article
- Keywords:
heat shock protein;
heat stress;
molecular hybridization;
osteosarcoma cells;
cDNA;
mRNA;
rats
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The induction of hsp70 mRNA in human osteosarcoma cells (HOS-8603) and intacl rats under heat stress was studied using hsp70 cDNA labeled with a-32P dATP. The results showed that the induction of hsp70 mRNA was evident in liver, lung, spleen and the most evident was found in brain when the core body temperature of rats was brought to 42℃ for 15 min. The induction of hsp70 mRNA in HOS-8603 was also significant when cells were cultured at 42℃ for 30 min. These results indicated that hsp70 mRNA could be induced both in vitro and in vivo under heat stress condition and the induction of hsp70 mRNA in intact rats was of tissue-specific fashion.
