Specific Binding of U72099E to Glucocorticoid Receptors in Synaptic Plasma Membrane from Rat Brain
- VernacularTitle:21—氨基类固醇U72099E与大鼠突触质膜糖皮质激素受体的特异结合
- Author:
Qinzhi GONG
;
Zuo GUO
;
Renbao XU
;
Yizhang CHEN
;
Cheng ZHU
;
Guangji ZHANG
- Publication Type:Journal Article
- Keywords:
receptors, glucocorticoid;
synaptic membranes;
corticosterone;
dexanethasone;
animal;
rats
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The present study was undertaken by radioligand binding assay to compare the binding properties of U72099E to cytosol and synaptic plasma membrane (SPM)preparations of rat brain. The results showed that the binding of [3H]Glucocorticoids ([3H]GC) was not displaced by U72099E in cytosol but was displaced in SPM with a Ki of 32.2?1.7?mol/L-1,indicating the existence of binding sites for U72099E in plasma membrane (most probably being glucocorticoid membrane receptors, GCMR). The Ki of U72099E is close to the concentrations needed to mimic the actions of it to protect against brain injury, suggesting that GCMR may mediate the beneficial effect of U72099E.
