P53 Gene Mutations in Human Seminomas Screened by Using Polymerase Chain Reaction Technique
- VernacularTitle:用聚合酶链反应方法检测人睾丸精原细胞瘤中的P53基因突变
- Author:
Dingwei YE
;
Jiafu ZHENG
;
Songxi QIAN
;
Yongjiang MA
- Publication Type:Journal Article
- Keywords:
testicular neoplasms;
mutation;
oncogenes
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Genomic DNA from 17 primary human tcsticular seminomas was screend for the presence of mutations in exon 5 - 8 of P53 gene, using the single strand conformation polymorphism assay, followed by direct DNA sequencing. P53 mutations in one alle'e leading to an amino acid change were identified in 4 of 17 seminomas. Sites of mutations were in exon 5(codon 141), exon 7 (codon 238, codon 258) and exon 8(codon 279). The present study suggests that mutation of P53 gene is involved in the development of human testicular seminoma.
