Detection of Fetal Cells in Maternal Peripheral Blood by Polymerase Chain Reaction
- VernacularTitle:PCR技术检测孕妇外周血中胎儿细胞的研究
- Author:
Qi ZHANG
;
Xianghua LIN
;
Yumin MAO
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
fetal cells;
prenatal diagnosis
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
According to that Y-chromosomal sequence in characterised by Y-specific repeat DNA family (DYZ1 ), which contain 800-5000 copies, a pair of primers Y3, Y4 is designed to amplify their 446bp long of specific DNA segment by polymerase chain reaction (PCR), so as to detect male fetal cells in materal blood. In this paper male fetal cells in maternal blood can be detected by PCR amplification of unpurified DNA from maternal peripheral blood during various stages of gestation (early, middle, late). Compared with villi, ammotic fluid and deliverd neonate sex their coincident rate are 93%. 100%, 87. 5% respectively among three periods. It is revealed that noninvasive examining fetal cells from peripheral blood of pregnant women for diagnosis of sex-linked inherited diseases is significant valuable.
