Amplification of Hepatitis C Viral RNA 5 -Untranslated Region By Nested PCR
- VernacularTitle:用高度保守区引物和套式PCR扩增丙型肝炎病毒
- Author:
Shaotang REN
;
Yizhong QIN
;
Weiyie WANG
;
Guozhao ZHANG
;
Ruiwen CHEN
- Publication Type:Journal Article
- Keywords:
hepatitis C virus;
polymerase chain reaction;
diagnosis
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Hepatitis C viral RNA in sera from 10 patients with post-transfusion hepatitis in Shanghai (China) were amplified By the nested PCR with primer sets deduced from the American prototype HCV RNA 5' -untranslated region. Eight of 10 cases (80%) were positive. And manipulations of PCR were simplified by combination of reverse transcription step and the first round PCR reaction. Our results indirectly proved that 5-untranslated region of HCV RNA of Shanghai showed a high degree of comservation and homology compared with isolates from some regions in the world.
