EFFE CT OF EXCESS DIETARY METHIONINE ON ~3H- LEUCINE INCORPORATION OF HEPATIC AND BRAIN TISSUE IN RATS
- VernacularTitle:过量蛋氨酸对大鼠肝、脑组织~3H-亮氨酸掺入量的影响
- Author:
Peizhen OUYANG
;
Yangbo TANG
- Publication Type:Journal Article
- Keywords:
methionine. 1H-leucine liver brain
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Fifty four male Wistar rats weighing 60-90g were divided into three groups of 18 each; rice-soybean basal diet with 0.2%DL-methionine as control, 2.0%D1-methionine supplemented group, 2.0%DL-methionine and 3.0%glycine supplemented group. All animals were housed in individual wire cages and both experimental diet and water were given ad libitum. After feeding 21 days, 0.037MBq/g bw of 3H-leucine was injected intraperitoneally. At 1,2, 4, 6, 8, 10 hours after injection, three rats per group were killed and the radioactivity of 3H-leucine per mg hepatic and brain tissue (dpm/mg tissue) were determined by liquid scintillation counter. The results showed that 3H-leuciue incorporarted during the entire 10 hours period of observation markedly declined and its peak was delayed in the hepatic tissue of excessive methionine supplemented group. However, 3H-leucine incorporated was improved significantly in liver in the rats fed excessive DL-methionine with glycine diet. In contrast, no significant difference of incorporation in the rat brain among the three groups were found. The results demonstrated that the addition of excess methionine retarded evidently protein synthesis of hepatocytes in the rats, but no similar toxic effect occurred in the rat brain tissue.
