Inhibitory Effects of Protein Kinase C Inhibitors on Polymorphoneuclear-endothelial Cell Adhesion
- VernacularTitle:蛋白激酶C抑制剂对白细胞-内皮细胞粘附的抑制作用
- Author:
Xiangbin YU
;
Dinghua QIAN
;
Junping ZHANG
- Publication Type:Journal Article
- Keywords:
endothelium, vascular;
tumor necrosis factor;
platelet activating factor;
protein kinase C;
leukocyte adhesion;
animal;
cattle
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of protein kinase C (PKC) inhibitors 1- (5-isoquinolinylsulfonyl) - 2-methylpoperazine (H-7) and quercetin on endotheliai-polymorphoneuclear (EC-PMN) adhesion induced by tumor necrosis factor (TNF) and platelet activating factor (PAF) were studied in cultured bovine pulmonary artery endothelial monolayers in vitro. TNF (100 U/ml) and PAF (1.0 ?mol/L) stimulated EC dependent PMN-EC adhesion. Both H-7 and quercetin dose-dependently inhibited TNF and PAF induced PMN-EC adhesion. The IC50 of H-7 was 22.22, 5.25 umol/L, and that of quercetin was 18.30, 4.83 ?mol/L respectively. WEB-2086, a specific PAF receptor antagonist, dose-dependently inhibited PAF induced PMN-EC adhesion, but had no effect on TNF induced adhesion. These results suggest that PKC play an important role in EC activation by TNF or PAF, and TNF induced PMN-EC adhesion by independent on endogenetic PAF.
