DETERMINATION OF TOTAL CHOLINE IN INFANT MILK POWDER AND FOODS
- VernacularTitle:婴儿奶粉及食品中胆碱的测定
- Author:
Lijuan ZHANG
;
Weiying LI
- Publication Type:Journal Article
- Keywords:
choline infant formula
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
The "free" and "bound" forms of cholinc in food were determined by a rapid method in which extraction and hydrolysis were accomplished simulta- neously with a barium hydroxide-methanol-chloroform mixture. The choline was isolated by adsorption on a Florisil column. By passing ammonium rei-neckate through the column, a pink band of choline reineckate was formed. The choline reineckate could be eluted by acetone and its concentration was determined by measuring the absorbance of eluate of the reineckate band by spectrophotometer at 526nm.This method is accurate, rapid, easy to handle. The RSD is 2.43%. The recoveries of added choline ranged from 96.7% to 101.7%.
