Inununocytochemical Localization Study of Copper-Zinc Superoxide Dismutase in Human Myocardium
- VernacularTitle:人心肌铜锌超氧化物歧化酶的免疫组化定位
- Author:
Yi ZHENG
;
Zhenhua LIU
;
Kaihua CAI
- Publication Type:Journal Article
- Keywords:
myocardium;
superoxide dismutases;
immunohistochemistry;
ultrastructure;
radioimmunoassay
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The distribution of copper-zinc superoxide dismutase (Cu-ZnSOD) in subcellular organelles in 8 cases of normal human myocardium was studied by using immunocytochemistry and immunoelectron microscopic technique and the contents of Cu-Zn SOD in myocardium were measured by radioimmunoassay. The results showed that Cu-ZnSOD was in most myocardial cells. The contents of Cu-ZnSOD in the ventricular myocardial cells were higher than those in the atrial myocardial cells. The Cu-ZnSOD labeled with immunogold particles were distributed throughout cytoplasm and nuclei, but less in mitochondria and a very little in lysosomes. No gold particle was found on the membranous structure of sarcoplasmic reticulum as well as Golgi complex. The results demonstrate that the myocardium was the tissue containing quite abundant Cu-Zn SOD.
