Enhancing Effect of Anti-tumor Antibody on the Cytotoxicities of Lymphokine- activated KiUer Cells
- VernacularTitle:抗瘤抗体对LAK细胞抗肿瘤活性的增强作用
- Author:
Guangwen CAO
;
Ping DU
- Publication Type:Journal Article
- Keywords:
antibody, tumor;
interleukin-2;
killer cells;
in vitro
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Adult Kunming mice were immunized with K562 and P815 by a single intraperitoneai injection at a dose of 5?104 cells/murine/d. Anti-P815 and anti-K562 antisera were prepared. Contents of anti-tumor antibodies in the antisera were proved to be high by means of ELISA assay. Splenocytes of C57BL/6 mice and peripheral blood lymphocytes (PBL) from cancer patients were incubated with 125-1000 U/ml IL-2 for 4d. Anti-P815 activity of the LAK cells from C57BL/6 splenocytes was greatly enhanced in the presence of anti-P815 anti-serum at the early stage of culture of LAK and P815 together in 18h [125I]UdR release assay. Anti-K562 activity of LAK cells from PBL was also greatly enlianced by anti-K562 antiserum when the antiserum was added at the early stage of culture. Anti-tumor antibodies in the antisera, not complement and other cytokines, were responsible for the augmentation of NK and LAK cell activities.
