Amplification of ZFY Gene for Determination of Fetal Sex by Polymerase Chain Reaction
- VernacularTitle:聚合酶链反应扩增ZFY基因诊断胎儿性别
- Author:
Xianghua LIN
;
Qi ZHANG
;
Yumin MAO
;
Shuqing YANG
- Publication Type:Journal Article
- Keywords:
polymerase chain reaction;
ZFY gene;
sex determination;
fetus
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
According to ZFY gene sequence on Y-chromosome, which supposedly codes for the testis determining factor (TDF), a pair of primers F3.2 and F4.2 were designed to specifically amplify 650 bp DNA fragment. The amplified product was electrophoresized on 1.5% agarose gel stained with EB, and then directly visualized under UV. This protocol resulted in constantly producing 650 bp band of the specific product by amplification of 500 pg male genomic DNA and had the same result when mixed with 100 fold amount of female genomic DNA. When this method was used to detect Y-ZFY of chorionic villi and amniotic fluid in 20 samples respectively, its accuracy for determining fetal sex reached 100 percent.
