Investigation on detection of specific antibodies with synthetic peptides corresponding to certain regions of hepatitis C virus antigen
- VernacularTitle:应用丙型肝炎病毒蛋白多区段合成肽检测特异性抗体的探讨
- Author:
Fei HAO
;
Mengdong LI
- Publication Type:Journal Article
- Keywords:
hepatitis viruses;
peptides/BI;
antibody response;
ELISA
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Four synthetic peptides,CP10,CP9,CP33c and CP100,with the sequences corresponding to the capsid protein and nonstructural protein of hepatitis C virus(HCV)respectively,were employed to establish ELISA to detect anti-HCV.Serum HCV RNA was identified with reverse transcriptive polymerase chain reaction(RT - PCR)for comparison.Out of 320 sera collected from patients with viral hepatitis,patients with other diseases and blood donors,49 were positive for antibodies against a single or several peptides.Among the positive rates of antibodies against a single peptide,anti-CP10 rate(9.69%)was the highest and anti-CP33c(3.44%)the lowest.The positive rate for anti-HCV antibodies was significantly higher when 4 peptides were combinedly used than when only one peptide(P
