Vincristine inhibits HL-60 Cells proliferation by interfering the metabolism of phosphoinositides
- VernacularTitle:长春新碱对HL—60细胞肌醇磷脂代谢和增殖的影响
- Author:
Bo WU
;
Nianci LIANG
- Publication Type:Journal Article
- Keywords:
vincristine;
phosphoinositide metabolism;
leukemia;
cell proliferation
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Human promyelocytic leukemia HL - 60 cells labelled with 3H - inositol were treated with vincristine (VCR), it was found that the radioactivity of 3H-phosphatidyl inositol - 4-phosphate (PIP) and 3H - Phosphatidyli-nositol - 4,5 - bisphosphate(PIP2) had no obvious change, but that of 3H-phosphatidyl inositol (PI) decreased rapidly, only accounted for 38. 55%~61.01% of the control during 1~ 120 min of the treatment. It showed that VCRdid not affect the activity of PI kinase and PIP kinase, but enhanced the hydrolysis of PI of HL - 60 cells. The inhibitorty effect of VCR upon cell proliferation and DNA synthesis was observed after above biochemical changes,these results suggest that VCR may inhibit HL-60 cells proliferation by interfering the metabolism of phosphoininositides.
