In Vitro Study of Differentiation of U_(937) Cells Induced by rhTNF-?, rhIL-1-?, PHA-LCM and c-RA
- VernacularTitle:rhTNF-?,rhIL-1-?,PHA-LCM和顺式维甲酸对U_(937)细胞诱导分化的体外研究
- Author:
Feng YAN
;
Minghua ZHANG
;
Rujun YANG
- Publication Type:Journal Article
- Keywords:
U937 cell line;
induction;
differentiation;
cytokine
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, several physiological factors were studied for their effects on the growth and differentiation of U937 cells in vitro .The results showed that (1)rhTNF-?,PHA-LCM and c-RA exerted a dose and time-dependent suppression of proliferation of U937 cells; (2) the synergistic effect oc-cured when rhTNF-? was combined with c-RA in the cell culture; and (3)rhIL-1-? augmented the differentiation of U937 cells induced by c-RA though it had no effect on the cell line by itself. According to the [3H]-TdR incorporation, it can be concluded that the reduction of DNA synthesis of U917 cells by rhTNF-? ,PHA-LCM,and c-RA is attributed to the inhibition of the growth. The results might have clinical significance in the induction of differentiation of leukemia cells.
