A study on point mutation of ras oncogenes
- VernacularTitle:ras癌基因点突变的研究
- Author:
Junru WANG
;
Guoren DENG
;
Weiwen LIU
- Publication Type:Journal Article
- Keywords:
oncogene;
mutation;
PCR-RELP
- From:Journal of Third Military Medical University
1984;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
It is known that no restriction endonuclease cleavage site exists at 12 codon in N-ras gene and at 12 and 13 codon in K-ras gene.Amismatched base was incorporated at the 3' end primer to creat a kind of restriction endonuclease cleavage site.Then it was possible for us to analyze the point mutation of the above mentioned codons with the polymerase chain reaction-restriction fragment length polymorphism.In this paper,the study of the point mutation at 12 and 61 codon in c-Ha-ras,at 12 codon in N-ras,and at 12 and 13 codon in K-ras was reported.
