STUDY ON METHOD OF PURIFYING 1,25-(OH)_2D_3- 26,23-LACTONE WITH ONE STEP OF HPLC
- VernacularTitle:1,25-(OH)_2D_3-26,23-Lactone的一步高压液相色谱纯化法
- Author:
Minci ZHENG
;
Lili GAO
- Publication Type:Journal Article
- Keywords:
HPLC straight-phase type column 1,25-(OH)2D3-26,23-lactone
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
An improved method for the purification of 1,25-(OH)2D3-26,23-lactone was established by using one step of highperformance liquid chromatography (HPLC). The analytic column was Shim-pack NH2 (straight-phase type). The mobile phase was Methanol: Dichloromethane: Hexane = 4:26:70 and the flow rate was 2ml/min. An ultraviolet detector with 254nm was used. The retention time of 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1, 24, 25-(OH)3D3 werel2.10, 17.06 and 22,00 minutes respectively. This method can separate 1,25-(OH)2D3, l,25-(OH)2D3-26,23-lactone and 1,24,25-(OH)3D3 only by one step of HPLC and it is better than the old one which need two steps of HPLC for the separation of these substances.