Priming Effect of Intraperitoneally Injected Astragalus, Acanthopanax senticosus and Lycium barbarum Polysaccharides on the Induction of Lymphokine- activated Killer Cells in Vitro
- VernacularTitle:黄芪多糖、刺五加多糖和枸杞多糖在体内对LAK细胞抗肿瘤活性的调节作用
- Author:
Guangwen CAO
;
Ping DU
- Publication Type:Journal Article
- Keywords:
polysaccharide;
interleukin-2;
killer cells;
iodine radioisotopes;
in vivo
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
A single intraperitoneal injection (ip) of Astragalus polysaccharide (APS), polysaccharide of Acanthopanax senticosus (PAS) and Lychtm barbamm polysaccharide (LBP) at a dose of up to 10 mg/kg body weight could render splenocytes of adult C57BL/6 mice proliferate significantly higher than normal saline (NS)control. Splenocytes of LBP treated aged mice were 4 times higher than those of NS control of aged mice. The splenocytes of the mice treated with APS, PAS, LBP and NS were incubated with 125 - 1 000 U/ml rIL-2 for 4d in vitro, respectively. The cytotoxicity of LAK cells from the splenocytes of APS, PAS and LBP treated adult mice, in 18 h [125I]UdR release assay, were 70% ~ 120%, 20% -90%, 26% ~ 80% higher than that of the NS control, respectively. The dose of rIL-2 was reduced 75%, 50%, 50%, respectively, in vitro. The cytolytic activities of LAK cells from the splenocytes of LBP treated aged mice were 120%-200% higher than those of the NS control, and the dosage of rIL-2 was decreased more than 75% in vitro.
