Experimental Study of Effectiveness of Hyperbaric Oxygen Combined with Silybin on Lschemic-reperfused Injured Limbs
- VernacularTitle:高压氧水飞蓟宾联合应用对兔肢体缺血再灌注损伤的治疗作用
- Author:
Yifan KANG
;
Jianzhang GAO
;
Yiqun FANG
;
Yaocheng RUI
;
Duxin SUN
;
Guoqian ZENG
- Publication Type:Journal Article
- Keywords:
reperfusion;
hyperbaric oxygen;
silybin;
rabbits
- From:
Academic Journal of Second Military Medical University
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
27 rabbits were divided into normal group(n = 4),control group (n = 5), silybin group (n = 6), hyperbaric oxygen (HBO) group (n = 6), and HBO combined with silybin group (n = 6). Circulation of hindlimb was interrupted completely for 6 h and reperfused for 1 h. Malondialdehyde (MDA) and superoxide dismutase(SOD) in plasma from ischemic-reperfused injured limb were measured. Adenosine triphosphate (ATP) and creatine phosphate (CP) in samples taken from anterior tibial muscle were determined. Ultrastructural changes of injured muscle were observed. The results showed that HBO combined with silybin treatment had a favourable effect on ischemic and reperfused injured limb muscle with reduction of the lipid peroxidated injury, increase of the SOD activity and the high energy phosphate compounds (ATP and CP), and a promoting recovery of injured muscle cells. HBO and silybin had a synergistic action. It suggests that HBO combined with silybin is an effective method for treatment of ischemic and reperfused injured limb.
