Effects of 1,25(OH)2D3 Analogue MC903 on Proliferation and Differentiation of a Hu man Megakaryoblastic Leukemia Cell Line
- VernacularTitle:1,25(OH)_2D_3类似物MC903对人原始巨核白血病细胞系生长分化的影响
- Author:
Liangnian SONG
;
Tao CHENG
- Publication Type:Journal Article
- Keywords:
1,25-dihydroxyvitamin D3;
receptor, leukemia
- From:
Academic Journal of Second Military Medical University
1985;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of MC903, a novel 1,25-dihydroxyvitamin D3 [1,25(OH)2 D3] analogue, on proliferation and differentiation of a human megakaryoblastic leukemia cell line (HIMeg) were investigated. MC903 was found to inhibit cell proliferation and induce cell differentiation of HIMeg cells. Colony formation assay showed that MC903 (10-10 to 10-6mol/L) almost completely inhibited the colony-forming ability of HIMeg cells. Cell cycle analysis demonstrated that MC903 altered the cell cycle distribution; the fraction of cells in G0 + G1 increased while those in S and G2 + M decreased after treatment with MC903 for 4d. The effect of MC903 on proliferation and differentiation of HIMeg cells was in a dose- and time-dependent manner and was more active than that of 1, 25(OH)2, D3,. The results of the present study suggested that 1,25(OH)2 D3 as well as its novel analogue MC903 might play an important role in modulating the proliferation and differentiation of megakaryocytic lineage.
