NADPH Diaphorase Staining Retinal Cells in Streptozotocin-induced Diabetic Rat Retina.
- Author:
Soo Jeong LEE
1
;
Kuhl HUH
Author Information
1. Department of Ophthalmology, College of Medicine, Korea University, Seoul. Korea.
- Publication Type:Original Article
- Keywords:
Diabetic rat retina;
NADPH diaphorase
- MeSH:
Animals;
Diabetic Retinopathy;
Injections, Intraperitoneal;
NADP*;
NADPH Dehydrogenase*;
Neurons;
Nitric Oxide;
Rats*;
Retina*;
Retinal Neurons;
Retinaldehyde*;
Synaptic Transmission
- From:Journal of the Korean Ophthalmological Society
1998;39(10):2377-2384
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Nitric oxide(NO) is a free radical which serves a wide variety of functions on vascular tone, neurotransmission, immune cytotoxicity, and many others. Nitric oxide synthase(NOS) is the biosynthetic enzyme of NO and colocalized with NADPH diaphorase(NADPH-d) activity in many tissues. The author aimed to assess the changes that occur in this populations of neurons in the streptozotocin-induced diabetic rat where the retinal vasculature is known to be dysfunctional. The 8 rats was a diabetic group and the other 8 was a control group. Diabetes was induced with a single intraperitoneal injection of streptozotocin(65mg/kg). Four weeks later, the retina was flat mounted and stained with NADPH-d. Counting of the stained cells was made. There was a 20.6% decrease in the total number of positively staining cells in the retinas of the diabetic group(2532+/-192) compared with those of the control group(3188+/-176)(p<0.001). It is worth to suggest the close correlation between NO released from retinal neurons and the microcirculatory dysfunction in diabetic retinopathy.
