Effects of Retinoic Acid on the Proliferation and Differentiation of Human Osteosarcoma Cells
- VernacularTitle:视黄酸对人骨肉瘤细胞增殖分化的影响
- Author:
Liangnian SONG
- Publication Type:Journal Article
- Keywords:
retinoic acid;
glucocorticoids;
osteosarcoma;
receptor;
alkaline phosphatase
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
The effects of retinoic acid (RA) on the proliferation and differentiation of a newly established human osteosarcoma cell line, HOS-8603, were studied. The results showed that RA could inhibit the proliferation of HOS-8603 cells at different concentrations ranging from 10-6 to 10-10 mol/L, and the inhibitory effect of RA was in a dose-dependent manner. In addition, RA (10-8 mol/L) could also significantly suppress the clonal proliferation of HOS-8603 cells. The effects of RA on the alkaline phosphatase activity in HOS-8603 cells were also determined. It was found that the alkaline phosphatase activity could be induced 48 h after treatment with RA (10-7 mol/L). The induction of alkaline phosphatase activity and morphological changes in HOS-8603 cells by RA suggested that RA might play roles in the differentiating processes. The interactions between retinoic acid and dexmathasone (Dex), a synthetic glucocorticoid, in the control of HOS-8603 cell proliferation were also investigated, and it was found that RA at low concentrations (10-8 to 10-10 mol/L) had a synergistic effect, while high concentrations of RA (10-6 and 10-7 mol/L) had a antagonistic effect with Dex on the proliferation of HOS-8603 cells.
