Comparative Studies on Kinetics of Mitochondrial LDH and Solubilized Mitochondrial LDH in Rat Liver
- VernacularTitle:大鼠肝线粒体LDH及溶脱线粒体LDH的动力学比较研究
- Author:
Jianxin SUN
;
Zhi CHEN
;
Yushan YANG
- Publication Type:Journal Article
- Keywords:
mitochondria;
lactate dehydrogenase;
liver, animal;
rats
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, we studied the kinetics of nitochondrial lactate dehydrogenase (LDH) and solubilized mitochondrial LDH in the rat liver. The apparent Km values of mitochondrial LDH and solubilized mitochondrial LDH for substrate pyruvate were 50.0 ?mol/L and 33.8 ?mol/L, and those for NADH were 35.3?mol/L and 21.4 ?mol/L, respectively. The apparent Km values of mitochondrial LDH were greater than those of solubilized nitochondrial LDH. The mitochondrial LDH in the rat liver was mainly LDH-5, which could be solubilized by 0.15 mol/L NaQ solution.
