Study of Monoclonal Antibody Against Sulfonated DNA
- VernacularTitle:抗磺化DNA单克隆抗体的研究
- Author:
Weiming SUN
;
Beihua DONG
;
Zhigong XU
;
Sikun YANG
;
Zhengfang ZHOU
;
Linli ZHENG
- Publication Type:Journal Article
- Keywords:
antibody, monoclonal;
DNA probes;
animal;
mice
- From:
Academic Journal of Second Military Medical University
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, 5'-CMP was sulfonated, and then the modified 5'-CMP was connected to a protein carrier as an immunogen to immunize BALB/c mice. After cell hybridization, screening and recloning , a McAb (B10) with high sensitivity and specificity was selected. In a dot Hot using the McAb B10, less than 0.05 pg of sulfonated DNA could be detected while 10 ng of DNA was not coloured The result showed that the sensitivity of McAb B10 was higher than that of the McAb from "Chemiprobe" kit
