Quantitative analysis of membrane phospholipids by TLC in ischemic - reperfused rat heart
- VernacularTitle:膜磷脂分离测定缺血再灌时大鼠心肌膜磷脂的变化
- Author:
Xiang FANG
;
Zhaogui QUO
- Publication Type:Journal Article
- Keywords:
Membrane phospholipids;
TLC;
Phosphorus quantitation;
Rat;
Ischemia/Reperfusion;
Myocardium
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Thin-Layer Chromatography (TLC)used for separation and quantitation of phospholipids including phosphatidyl-choline (PC), Phosphatidyl-ethanolamine (PE), platelet activating activating-factor (PAF) were studied. The experimental processes were: lipid extract from tissue sample by Bligh-dyer methods, separation of phospholipids by TLC. and quantitation of phospholipids by phosphorus quantitative method. Using this method had found that reperfusion caused membrane phospholipids degradation in rat heart in situ subjected to 5 min of ischemia and followed by 10 min of reperfusion, myocardial total phospholipid, PC, PE decreased by 38.7, 46. 2 and 42.2%. respectively. Results indicated that membrane phospholipid play an important role in the genesis of ischemic-reperfusion induced myocardial damage.
