Hemorrhagic Shock-induced Damage in the Inner Membrane of Rat Myocardial Mitochondria
- VernacularTitle:失血性休克致鼠心肌线粒体内膜的损伤
- Author:
Shanhong ZHU
;
Mingyong MIAO
;
Kerning CHEN
;
Hangping SHI
- Publication Type:Journal Article
- Keywords:
shock;
hemorrhagic;
mitochondria;
respiratory chain;
animal;
rats
- From:
Academic Journal of Second Military Medical University
1981;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
The alterations in electron transport were studied in the myocardial mitochondria of rats with hemorrhagjc shock. Hemorrhagic shock model was induced by a modified Wigger procedure. Mitochondria were obtained by differential centrifugation. Succinate-, and NADH-respiratory chains were assayed by polarographically and spectrophotometrically in isolated myocardial mitochondria. The results showed that hemorrhagic shock led to progressive decrease in the enzymatic activities of two respiratory chains. The activities of Succinate-Co. Q reductase, Succinate-Cyt. C reductase, NADH-Co. Q reductase, NADH-Cty. C reductase and cytochrome oxidase were remarkably lower in shock 3 h group than those in the shamoperated. This implies that there is not only low-flow hypoxia, but inability to utilizer oxygen in the myocardial mitochondria
